@article {1145, title = {Two new distribution records of Aedes (Rusticoidus) refiki Medschid, 1928 (Diptera: Culicidae) from Germany}, journal = {Journal of the European Mosquito Control Association}, volume = {35}, year = {2017}, month = {12/05/2017}, pages = {18-24}, abstract = {Although relatively rare, the culicid species Aedes refiki is considered widely distributed throughout Europe. It has also been described to occur over large parts of Germany, but reports are scarce and date back several decades. The last time Ae. refiki was documented for Germany was in 1980 when the species was found in the central northern part of the country. During larval sampling activities, Ae. refiki was rediscovered at two locations in the German federal state of Thuringia in spring 2016. The collection sites, method of species identification and species characteristics are described and discussed. Journal of the European Mosquito Control Association 35: 18-24, 2017}, keywords = {Aedes refiki, Culicidae, ecology, Germany, identification}, author = {Kuhlisch, Cornelius and Kampen, Helge and Walther, Doreen} } @article {1145, title = {Species identification of Swedish mosquitoes through DNA metabarcoding}, journal = {Journal of the European Mosquito Control Association}, volume = {35}, year = {2017}, month = {16/02/2016}, pages = {1-9}, abstract = {Abstract: DNA-barcoding utilises a fragment of the mitochondrial cytochrome oxidase subunit 1 (COI) gene to identify most animal species. Using next generation sequencing (NGS), this method can be further developed into metabarcoding processes that allow the simultaneous identification of several species from a mixed sample. We created a database of COI sequences of 27 mosquito species collected in Sweden, and combined our data with 27 additional sequences from GenBank to cover the taxa recently documented in Sweden and to include possible invasive taxa. Comparisons show that COI metabarcoding reliably identifies 41 of 54 species and the remainder to species group. Using three independent primer pairs along the COI gene, we further developed this barcoding approach to simultaneously identify Swedish mosquitoes in communities using NGS and quantify relative abundance of each mosquito species in the sample, using bioinformatics methods. We tested the accuracy of the metabarcoding method using communities assembled from morphologically identified mosquitoes, revealing 80\% positive identification rate and the estimates of population structure which reflects the input sample. We conclude that metabarcoding is useful as a high throughput identification technique and for the quantification of species. Journal of the European Mosquito Control Association 35: 1-9, 2017}, keywords = {COI, Culicidae, metabarcoding, next generation sequencing, surveillance, vectors}, author = {Lilja, Tobias and Nylander, Johan A.A and Troell, Karin and Lindstr{\"o}m, Anders} } @article {1145, title = {Blood-feeding Diptera (Culicidae and Ceratopogonidae) in an urban park of the city of Vitoria-Gasteiz (Basque Country, Spain)}, journal = {Journal of the European Mosquito Control Association}, volume = {33}, year = {2015}, month = {06/07/2015}, pages = {10-14}, abstract = {Haematophagous and synanthropic Diptera are responsible for the transmission of several pathogenic agents to humans, domestic and wild animals, causing diverse vector-borne diseases of paramount importance. The presence of these blood-feeding insects is well reported in many areas of the world; however, the distribution in anthropogenic zones has not been studied so far across Spain. In order to gather information on this entomofauna in urban environments, adult stages were collected using an automatic entomological aspirator during the summer season in street lamps of a public park located in the city of Vitoria-Gasteiz (Basque Country, Spain). A total of 333 culicids from four species were collected in a two-year sampling period: Culex pipiens (91.3\%), Culiseta longiareolata (8.1\%), Culiseta litorea (0.3\%) and Culex hortensis hortensis (0.3\%). Moreover, 225 Culicoides specimens belonging to six different species were trapped: Culicoides punctatus (61.3 \%), Culicoides obsoletus s.s. (16.8\%), Culicoides duddingstoni (13.3\%), Culicoides kibunensis (6.2\%), Culicoides dewulfi (1.7\%) and Culicoides clastrieri (0.4\%). The first data of Culicoides species distribution in urban areas and the first record of C. litorea are recorded for the Basque Country region. }, keywords = {Basque Country, biting midges, Culicidae, Culicoides, mosquitoes, Spain, urban environment}, author = {Gonzalez, M and Lopez,S and Alarcon-Elbal, PM} } @article {1135, title = {Invertebrates found in underground shelters of western Bohemia. I. Mosquitoes (Diptera: Culicidae)}, journal = {Journal of the European Mosquito Control Association}, volume = {32}, year = {2014}, month = {19/12/2014}, pages = {27-32}, abstract = {

Mosquitoes found hibernating in underground shelters were studied in western Bohemia, Czech Republic. Species collected included Culiseta alaskaensis, Cs. annulata, Cs. glaphyroptera, Culex spp., and Anopheles maculipennis s.l.. Culex spp. were the most common, followed by Cs. glaphyroptera and Cs. annulata with few records of Cs. alaskaensis and An. maculipennis s.l.. Culex spp. were found in a variety of shelters, An. maculipennis s.l. preferred cellars, and Cs. alaskaensis where they were found, exhibited little preference. Culiseta annulata and Cs. glaphyroptera were found in similar numbers of cellars, caves, and bunkers, but Cs. glaphyroptera were found more commonly in mines than Cs. annulata.


}, keywords = {Anopheles, Culex, Culicidae, Culiseta, Czech Republic, hibernation, underground shelters}, author = {Dvorak, Libor} }