@article {1145, title = {Pathways for introduction and dispersal of invasive Aedes mosquito species in Europe: a review}, journal = {Journal of the European Mosquito Control Association}, volume = {38}, year = {2020}, month = {17/12/20}, pages = {1-10}, abstract = {Abstract: In the last decades, Aedes invasive mosquito species (AIMs) have arrived in Europe from overseas, introduced via different pathways. Several species such as Aedes albopictus, Ae. japonicus and Ae. koreicus were introduced, built populations, and expanded their distribution into new regions. The introduction and establishment of AIMs in Europe is a risk to public health, due to the ability of these mosquitoes to transmit diverse pathogens of vector-borne diseases. The objective of this manuscript is to review knowledge of pathways associated with the introduction and dispersal of AIMs in Europe. The identification of pathways for introduction of AIMs is critical to decide on surveillance strategies needed to reduce the risk and control future introductions. Four main routes are identified and discussed: the passive transport of AIMs via (1) the trade of used tyres, (2) the trade of Lucky bamboo plant cuttings, (3) vehicles (traffic by road, airplanes, and sea), as well as (4) the active natural dispersal of AIMs. We conclude that the trade of used tyres remains the main pathway for long distance transportation and introduction of AIMs into and within Europe. Furthermore, passive transport by road in ground vehicles (e.g. car, truck) represents the major driving force for dispersion from already established populations. Journal of the European Mosquito Control Association 38: 1 {\textendash} 10, 2020}, keywords = {Aedes, dispersal, Europe, invasive mosquitoes, pathway, surveillance, vector-borne diseases}, author = {A. Iba{\v n}ez-Justicia} } @article {1145, title = {Species identification of Swedish mosquitoes through DNA metabarcoding}, journal = {Journal of the European Mosquito Control Association}, volume = {35}, year = {2017}, month = {16/02/2016}, pages = {1-9}, abstract = {Abstract: DNA-barcoding utilises a fragment of the mitochondrial cytochrome oxidase subunit 1 (COI) gene to identify most animal species. Using next generation sequencing (NGS), this method can be further developed into metabarcoding processes that allow the simultaneous identification of several species from a mixed sample. We created a database of COI sequences of 27 mosquito species collected in Sweden, and combined our data with 27 additional sequences from GenBank to cover the taxa recently documented in Sweden and to include possible invasive taxa. Comparisons show that COI metabarcoding reliably identifies 41 of 54 species and the remainder to species group. Using three independent primer pairs along the COI gene, we further developed this barcoding approach to simultaneously identify Swedish mosquitoes in communities using NGS and quantify relative abundance of each mosquito species in the sample, using bioinformatics methods. We tested the accuracy of the metabarcoding method using communities assembled from morphologically identified mosquitoes, revealing 80\% positive identification rate and the estimates of population structure which reflects the input sample. We conclude that metabarcoding is useful as a high throughput identification technique and for the quantification of species. Journal of the European Mosquito Control Association 35: 1-9, 2017}, keywords = {COI, Culicidae, metabarcoding, next generation sequencing, surveillance, vectors}, author = {Lilja, Tobias and Nylander, Johan A.A and Troell, Karin and Lindstr{\"o}m, Anders} } @article {1139, title = {First interception of Aedes (Stegomyia) albopictus in Lucky bamboo shipments in Belgium.}, journal = {Journal of the European Mosquito Control Association}, volume = {32}, year = {2014}, month = {05/2014}, pages = {14-16}, chapter = {14}, abstract = {

Abstract: Six gel-and five water-transported Dracaena braunii shipments originating from the South coast of China were screened for exotic mosquito species during a surveillance project on exotic vectors in Belgium. In November 2013, a live Aedes (Stegomyia) albopictus larva was detected in a gel substrate. This is the first direct evidence of the importation of Ae. albopictus on gel-transported Lucky bamboo. It also confirms that the importation risk of Ae. albopictus by transport of ornamental bamboo plants remains. In addition to the registration of appropriate biocides, a structured and permanent surveillance programme is needed in Belgium to allow for the early detection of invasive mosquito species and the timely implementation of control measures. Journal of the European Mosquito Control Association 32: 14-16, 2014

}, keywords = {Aedes albopictus, Belgium, biocide legislation, Dracaena braunii, surveillance, tiger mosquito}, author = {Julie Demeulemeester and Isra Deblauwe and Jacobus De Witte and Famke Jansen and Adam Hendy and Maxime Madder} }