01434nas a2200157 4500008004100000245014100041210006900182260001200251300000900263490000700272520079900279100002501078700001601103700002301119856013401142 2002 eng d00aMolecular confirmation of sympatric populations of An. messeae and An. atroparvus overwintering in Kent, southeast England0 aMolecular confirmation of sympatric populations of iAn messeaei  c08/2002 a8-160 v133 a

Mosquitoes of the Anopheles maculipennis complex were collected as overwintering adults in a disused war fortification near the Cliffe marshes in the county of Kent, southeast England in January 2002. Fifty-three adult females were collected and fifty-two of these were identified as either An. atroparvus van Thiel or An. messeae Falleroni on the basis of similarity to rDNA internal transcribed spacer (ITS2) sequences in GenBank. Forty specimens (76.9%) were identified as An. messeae and 12 (23.1%) as An. atroparvus. DNA sequences for these species from England are provided for the first time, which enhance knowledge of the species composition and distribution of the An. maculipennis complex in England.

1 aLinton, Yvonne-Marie1 aSmith, Lisa1 aHarbach, Ralph, E. uhttps://e-m-b.myspecies.info/content/molecular-confirmation-sympatric-populations-ian-messeaei-and-ian-atroparvusi-overwintering-01265nas a2200157 4500008004100000245012000041210006900161260001200230300000800242490000700250520065200257100002500909700001600934700002300950856013400973 2002 eng d00aObservations on the taxonomic status of Anopheles subalpinus Hackett & Lewis and An. Melanoon Hackett0 aObservations on the taxonomic status of iAnopheles subalpinusi H c08/2002 a1-70 v133 a

Progeny broods of An. maculipennis s.l. from Greece were identified as An. subalpinus Hackett & Lewis based on egg morphology and the original species descriptions. DNA sequence data from members of the progeny broods were compared to previously published sequences for members of the An. maculipennis complex. The sequences were 99.54-100% identical to those of An. melanoon Hackett, typified by its uniformly black egg. These results are discussed in relation to those of other workers, and An. subalpinus is formally synonymised with An. melanoon.

1 aLinton, Yvonne-Marie1 aSmith, Lisa1 aHarbach, Ralph, E. uhttps://e-m-b.myspecies.info/content/observations-taxonomic-status-ianopheles-subalpinusi-hackett-lewis-and-ian-melanooni-hackett01378nas a2200169 4500008004100000245013000041210006900171260001200240300001000252490000700262520070900269100002500978700003201003700001601035700002301051856013401074 2001 eng d00aNew occurrence records for Anopheles maculipennis and An. messeae in northern Greece based on DNA sequence data0 aNew occurrence records for iAnopheles maculipennisi and iAn mess c12/2001 a31-360 v113 a

Bloodfed Anopheles maculipennis s.l. females were collected resting in and around sheepfolds in the northwestern prefecture of Florina, Greece. The specimens were identified as An. maculipennis and An. messeae on the basis of similarity to published DNA sequence data for the rDNA internal transcribed spacer (ITS2) region. Novel DNA sequences were obtained for the mitochondrial cytochrome c oxidase I gene (COl) for both species. Correlations of COl and ITS2 sequences were used to identitfy specimens, with respect to ITS2 sequences in Genbank. These results constitute new distribution records for these two species in northwestern Greece.

1 aLinton, Yvonne-Marie1 aSamanidou-Voyadjoglou, Anna1 aSmith, Lisa1 aHarbach, Ralph, E. uhttps://e-m-b.myspecies.info/content/new-occurrence-records-ianopheles-maculipennisi-and-ian-messeaei-northern-greece-based-dna-s