02067nas a2200217 4500008004100000245009900041210006900140260001300209300000900222490000700231520142900238653001001667653001401677653001101691653002401702653001201726653001701738653002601755100002501781856004301806 2020 eng d00aPathways for introduction and dispersal of invasive Aedes mosquito species in Europe: a review0 aPathways for introduction and dispersal of invasive Aedes mosqui c17/12/20 a1-100 v383 aAbstract: In the last decades, Aedes invasive mosquito species (AIMs) have arrived in Europe from overseas, introduced via different pathways. Several species such as Aedes albopictus, Ae. japonicus and Ae. koreicus were introduced, built populations, and expanded their distribution into new regions. The introduction and establishment of AIMs in Europe is a risk to public health, due to the ability of these mosquitoes to transmit diverse pathogens of vector-borne diseases. The objective of this manuscript is to review knowledge of pathways associated with the introduction and dispersal of AIMs in Europe. The identification of pathways for introduction of AIMs is critical to decide on surveillance strategies needed to reduce the risk and control future introductions. Four main routes are identified and discussed: the passive transport of AIMs via (1) the trade of used tyres, (2) the trade of Lucky bamboo plant cuttings, (3) vehicles (traffic by road, airplanes, and sea), as well as (4) the active natural dispersal of AIMs. We conclude that the trade of used tyres remains the main pathway for long distance transportation and introduction of AIMs into and within Europe. Furthermore, passive transport by road in ground vehicles (e.g. car, truck) represents the major driving force for dispersion from already established populations. Journal of the European Mosquito Control Association 38: 1 – 10, 202010aAedes10adispersal10aEurope10ainvasive mosquitoes10apathway10asurveillance10avector-borne diseases1 aIbaňez-Justicia, A. uhttps://e-m-b.myspecies.info/node/116802071nas a2200241 4500008004100000245007500041210006900116260001500185300000800200490000700208520138700215653000801602653001401610653001801624653003101642653001701673653001201690100001801702700002501720700001801745700002301763856004301786 2017 eng d00aSpecies identification of Swedish mosquitoes through DNA metabarcoding0 aSpecies identification of Swedish mosquitoes through DNA metabar c16/02/2016 a1-90 v353 aAbstract: DNA-barcoding utilises a fragment of the mitochondrial cytochrome oxidase subunit 1 (COI) gene to identify most animal species. Using next generation sequencing (NGS), this method can be further developed into metabarcoding processes that allow the simultaneous identification of several species from a mixed sample. We created a database of COI sequences of 27 mosquito species collected in Sweden, and combined our data with 27 additional sequences from GenBank to cover the taxa recently documented in Sweden and to include possible invasive taxa. Comparisons show that COI metabarcoding reliably identifies 41 of 54 species and the remainder to species group. Using three independent primer pairs along the COI gene, we further developed this barcoding approach to simultaneously identify Swedish mosquitoes in communities using NGS and quantify relative abundance of each mosquito species in the sample, using bioinformatics methods. We tested the accuracy of the metabarcoding method using communities assembled from morphologically identified mosquitoes, revealing 80% positive identification rate and the estimates of population structure which reflects the input sample. We conclude that metabarcoding is useful as a high throughput identification technique and for the quantification of species. Journal of the European Mosquito Control Association 35: 1-9, 201710aCOI10aCulicidae10ametabarcoding10anext generation sequencing10asurveillance10avectors1 aLilja, Tobias1 aNylander, Johan, A.A1 aTroell, Karin1 aLindström, Anders uhttps://e-m-b.myspecies.info/node/115201658nas a2200265 4500008004100000245009300041210006900134260001200203300001000215490000700225520088300232653002101115653001201136653002401148653002101172653001701193653001901210100002601229700001901255700002201274700001801296700001601314700001901330856004301349 2014 eng d00aFirst interception of Aedes (Stegomyia) albopictus in Lucky bamboo shipments in Belgium.0 aFirst interception of Aedes Stegomyia albopictus in Lucky bamboo c05/2014 a14-160 v323 a
Abstract: Six gel-and five water-transported Dracaena braunii shipments originating from the South coast of China were screened for exotic mosquito species during a surveillance project on exotic vectors in Belgium. In November 2013, a live Aedes (Stegomyia) albopictus larva was detected in a gel substrate. This is the first direct evidence of the importation of Ae. albopictus on gel-transported Lucky bamboo. It also confirms that the importation risk of Ae. albopictus by transport of ornamental bamboo plants remains. In addition to the registration of appropriate biocides, a structured and permanent surveillance programme is needed in Belgium to allow for the early detection of invasive mosquito species and the timely implementation of control measures. Journal of the European Mosquito Control Association 32: 14-16, 2014
10aAedes albopictus10aBelgium10abiocide legislation10aDracaena braunii10asurveillance10atiger mosquito1 aDemeulemeester, Julie1 aDeblauwe, Isra1 aDe Witte, Jacobus1 aJansen, Famke1 aHendy, Adam1 aMadder, Maxime uhttps://e-m-b.myspecies.info/node/1139