01424nas a2200229 4500008004100000245012800041210006900169260001200238300001000250490000700260520071400267653003100981653001001012653001901022653000901041100002401050700001801074700002201092700001801114700001901132856004301151 2017 eng d00aFirst monitoring of mosquito species (Diptera: Culicidae) in the Caffarella Valley, Appia Antica Regional Park, Rome, Italy0 aFirst monitoring of mosquito species Diptera Culicidae in the Ca c18/7/17 a29-320 v353 aThis study reports the results of the first entomological investigation focused on mosquitoes in Caffarella Valley, the inner part of Appia Antica Natural Reserve in Rome, carried out between 2012 and 2013. A total of 1173 mosquitoes were collected, with 9 species, belonging to 4 different genera, identified: Culex pipiens, Anopheles maculipennis sensu stricto (s.s.), Anopheles claviger, Culiseta annulata, Culiseta longiareolata, Aedes albopictus, Culex hortensis, Culex territans and Anopheles plumbeus. The monitoring of this area, bordering natural and urban environments, contributes to the knowledge on the culicid fauna of Rome. Journal of the European Mosquito Control Association 35: 29-32, 2017 10aAppia Antica Regional Park10aItaly10amosquito fauna10aRome1 aSeverini, Francesco1 aToma, Luciano1 aPiccari, Fabrizio1 aRomi, Roberto1 aDi Luca, Marco uhttps://e-m-b.myspecies.info/node/115801492nas a2200253 4500008004100000245012100041210006900162260001500231300001000246490000700256520071200263653002100975653002500996653001201021653001001033653002501043100001801068700001901086700002701105700002001132700002401152700001901176856004301195 2017 eng d00aFirst record of Aedes albopictus (Skuse, 1894) (Diptera; Culicidae) from three islands in the Tyrrhenian Sea (Italy)0 aFirst record of Aedes albopictus Skuse 1894 Diptera Culicidae fr c05/07/2017 a25-280 v353 aAedes (Stegomyia) albopictus, an invasive mosquito originating from the Asian-Pacific region, is known as an important vector of several pathogens of public and veterinary health significance. In 1990, this mosquito was recorded for the first time in Italy (Port of Genova) and in about 20 years it has become established throughout the whole country. In this paper, we report for the first time the presence of Ae. albopictus on three small islands in the Tyrrhenian Sea, namely Giglio, Ventotene and Ustica. Most probably, the maritime transport of goods and tourists during the summer is the major entry route of this mosquito species. Journal of the European Mosquito Control Association 35: 25-28, 201710aAedes albopictus10aAsian tiger mosquito10aislands10aItaly10aMediterranean Basin.1 aToma, Luciano1 aToma, Federico1 aPampiglione, Guglielmo1 aGoffredo, Maria1 aSeverini, Francesco1 aDi Luco, Marco uhttps://e-m-b.myspecies.info/node/115701339nas a2200217 4500008004100000245011200041210006900153260001500222300001000237490000700247520068800254653001700942653001400959653001200973653001200985653001900997100002401016700001801040700002001058856004301078 2017 eng d00aTwo new distribution records of Aedes (Rusticoidus) refiki Medschid, 1928 (Diptera: Culicidae) from Germany0 aTwo new distribution records of Aedes Rusticoidus refiki Medschi c12/05/2017 a18-240 v353 aAlthough relatively rare, the culicid species Aedes refiki is considered widely distributed throughout Europe. It has also been described to occur over large parts of Germany, but reports are scarce and date back several decades. The last time Ae. refiki was documented for Germany was in 1980 when the species was found in the central northern part of the country. During larval sampling activities, Ae. refiki was rediscovered at two locations in the German federal state of Thuringia in spring 2016. The collection sites, method of species identification and species characteristics are described and discussed. Journal of the European Mosquito Control Association 35: 18-24, 201710aAedes refiki10aCulicidae10aecology10aGermany10aidentification1 aKuhlisch, Cornelius1 aKampen, Helge1 aWalther, Doreen uhttps://e-m-b.myspecies.info/node/115602244nas a2200217 4500008004100000245019100041210006900232260001300301300001000314490000700324520153100331653002101862653001301883653001201896653001101908653001701919653001101936100001801947700001801965856004301983 2017 eng d00aSpatial distribution of immature stages of Aedes albopictus (Skuse) (Diptera: Culicidae) in flower pots in a Spanish cemetery and field evaluation of metallic copper as a control agent0 aSpatial distribution of immature stages of Aedes albopictus Skus c2/5/2017 a13-170 v353 aCemeteries have been shown to permit the development of the invasive mosquito Aedes albopictus in Southern Europe and this has facilitated its establishment on account of the huge quantity of flower pots which are adequate breeding sites for this container-adapted species. A control technique consisting of the use of pieces of metallic copper has been repeatedly proposed as a control solution for mosquito larvae in these pots. Although theoretically promising, this technique has not been used at an operative level, as there has been a lack of information on the best adapted copper formulation as well as on the mosquito larval population dynamics. The present study was planned to characterise the spatial larval distribution of Aedes albopictus in flower pots in a wall-niche burial system, and to test the cost-effectiveness of metallic copper application. We found that the preliminary distribution of larvae and pupae in the cemetery was not related to height from the ground but varied between buildings. During the 8 weeks of the trial, application of copper in the form of thin electric wire resulted in a season-accumulated reduction of 90.95% in the production of larvae and 97.06% of the pupae. Application costs and social interactions are discussed as other control methodologies would be more cost-efficient in this specific context. Copper application could be suitable for domestic environments, if application is performed adequately. Journal of the European Mosquito Control Association 35: 13-17, 201710aAedes albopictus10acemetery10acontrol10acopper10adistribution10alarvae1 aEritja, Roger1 aHerreros, Eva uhttps://e-m-b.myspecies.info/node/115500633nas a2200193 4500008004100000245006800041210006700109260001500176300001000191490000700201520007300208653002700281653002300308653001200331653001700343653001300360100002300373856004300396 2017 eng d00aHistory of human-biting Culex pipiens in Sweden and Scandinavia0 aHistory of humanbiting Culex pipiens in Sweden and Scandinavia c21/03/2017 a10-120 v353 aJournal of the European Mosquito Control Association 35: 10-12, 201710aCulex pipiens molestus10aCulex pipiens s.l.10ahistory10aScandinavia.10ataxonomy1 aLindström, Anders uhttps://e-m-b.myspecies.info/node/115402071nas a2200241 4500008004100000245007500041210006900116260001500185300000800200490000700208520138700215653000801602653001401610653001801624653003101642653001701673653001201690100001801702700002501720700001801745700002301763856004301786 2017 eng d00aSpecies identification of Swedish mosquitoes through DNA metabarcoding0 aSpecies identification of Swedish mosquitoes through DNA metabar c16/02/2016 a1-90 v353 aAbstract: DNA-barcoding utilises a fragment of the mitochondrial cytochrome oxidase subunit 1 (COI) gene to identify most animal species. Using next generation sequencing (NGS), this method can be further developed into metabarcoding processes that allow the simultaneous identification of several species from a mixed sample. We created a database of COI sequences of 27 mosquito species collected in Sweden, and combined our data with 27 additional sequences from GenBank to cover the taxa recently documented in Sweden and to include possible invasive taxa. Comparisons show that COI metabarcoding reliably identifies 41 of 54 species and the remainder to species group. Using three independent primer pairs along the COI gene, we further developed this barcoding approach to simultaneously identify Swedish mosquitoes in communities using NGS and quantify relative abundance of each mosquito species in the sample, using bioinformatics methods. We tested the accuracy of the metabarcoding method using communities assembled from morphologically identified mosquitoes, revealing 80% positive identification rate and the estimates of population structure which reflects the input sample. We conclude that metabarcoding is useful as a high throughput identification technique and for the quantification of species. Journal of the European Mosquito Control Association 35: 1-9, 201710aCOI10aCulicidae10ametabarcoding10anext generation sequencing10asurveillance10avectors1 aLilja, Tobias1 aNylander, Johan, A.A1 aTroell, Karin1 aLindström, Anders uhttps://e-m-b.myspecies.info/node/115201611nas a2200229 4500008004100000245010800041210006900149260001500218300001000233490000700243520088600250653002101136653001901157653001801176653003001194653002001224100002101244700002301265700002301288700002701311856004301338 2016 eng d00aIntraspecific competition among larvae of Aedes albopictus in conditions of food abundance and shortage0 aIntraspecific competition among larvae of Aedes albopictus in co c12/12/2016 a14-160 v343 aAbstract: The competition between larvae of Aedes albopictus (Skuse, 1987), an invasive mosquito species recently established in Italy, was evaluated in laboratory in conditions of food abundance and shortage. The number of emerging adults, the time of emergence and the size of the adult bodies were recorded and compared. The number of adults that emerge under conditions of food abundance was found to be significantly higher than under food shortage. When food was lacking more males than females emerged and there was no significant difference in their body sizes, while under food abundance females were larger than males. Both males and females with abundant food were on average bigger than under food shortage. There was no difference in the time of adult emergence in the two different food conditions. Journal of the European Mosquito Control Association 34: 14-16, 201610aAedes albopictus10afood abundance10afood shortage10aIntraspecific competition10amosquito larvae1 aMacchioni, Fabio1 aChiavacci, Daniele1 aBiasci, Alessandro1 aPrati, Maria, Cristina uhttps://e-m-b.myspecies.info/node/115102484nas a2200301 4500008004100000245012600041210006900167260001500236300001000251490000700261520157200268653001001840653001201850653001701862653002401879653001301903653001401916100002601930700002601956700002001982700002402002700002402026700002102050700002102071700002302092700002402115856004302139 2016 eng d00aRediscovery of Aedes cretinus (Edwards, 1921) (Diptera; Culicidae) in Cyprus, 66 years after the first and unique report.0 aRediscovery of Aedes cretinus Edwards 1921 Diptera Culicidae in c17/11/2016 a10-130 v343 aAbstract: Mosquitoes are important vectors of parasitic helminths, protozoan and viral pathogens causing human disease. There has been a growing interest in the establishment and spread of invasive aedine mosquito species but also in understanding native mosquitoes. Following concerns regarding black and white-striped mosquitoes biting aggressively during the day, survey visits were organised by the Joint Services Health Unit on the Kryos river at the village of St. Mavra, Limassol district, Cyprus. Adult mosquitoes collected either during human landing catches in June 2015 or adults that emerged in the laboratory from water collected from tree holes in January 2016, were identified as Aedes (Stegomyia) cretinus. During the same period (June 2015), Ae. cretinus adults were also collected by the Public Health authorities of the Republic of Cyprus from a local residence at Avdellero village, Larnaca district. Aedes cretinus is a species with limited distribution and close resemblance to the Asian tiger mosquito Aedes (Stegomyia) albopictus. Information on mosquito species and their respective geographic distribution is fundamental for the goal of reducing the impact of emerging vector-borne viral zoonoses in Europe and targeting control for vector and nuisance species. Our study highlights the need for educational and raising awareness programmes regarding insects of medical importance as well as the importance of collaboration and networking between the relevant authorities. Journal of the European Mosquito Control Association 34: 10-13, 201610aAedes10aCyprus.10adistribution10aMediterranean basin10aMosquito10aStegomyia1 aMartinou, Angeliki, F1 aVaux, Alexander, G.C.1 aBullivant, Glen1 aCharilaou, Pantelis1 aHadjistyllis, Haris1 aShawcross, Kevin1 aViolaris, Marios1 aSchaffner, Francis1 aMedlock, Jolyon, M. uhttps://e-m-b.myspecies.info/node/115001497nas a2200301 4500008004100000245011200041210006900153260001500222300000800237490000700245520059700252653002100849653002100870653001700891653001700908653002200925653001200947653001100959100001800970700002900988700002101017700003001038700001401068700002201082700002001104700002801124856004301152 2016 eng d00aFirst record of Aedes albopictus (Skuse, 1894) (Diptera; Culicidae) from Minorca (Balearic Islands, Spain).0 aFirst record of Aedes albopictus Skuse 1894 Diptera Culicidae fr c29/09/2016 a5-90 v343 aAbstract: The Asian tiger mosquito is an invasive species showing a continuous expansion in the Mediterranean basin in general and in Spain in particular. The first detection of Aedes albopictus in mainland Spain was reported in 2004. Eight years later, in 2012, this mosquito species was detected in the Balearic archipelago, specifically on Majorca. In 2014 it was detected on Eivissa, another island from this archipelago. In this paper we report for the first time the presence of Ae. albopictus on the island of Minorca. Journal of the European Mosquito Control Association 34: 5-9, 201610aAedes albopictus10aBalearic Islands10aentry routes10afirst record10ainvasive mosquito10aMinorca10aSpain.1 aBengoa, Mikel1 aDelacour-Estrella, Sarah1 aBarceló, Carlos1 aParedes-Esquivel, Claudia1 aLeza, Mar1 aLucientes, Javier1 aMolina, Ricardo1 aMiranda, Miguel, Ángel uhttps://e-m-b.myspecies.info/node/114901567nas a2200205 4500008004100000245004900041210004900090260001500139300000800154490000700162520100600169653002201175653001801197653001101215653002201226653002701248100002101275700002201296856004301318 2016 eng d00aLaboratory colonisation of Aedes geniculatus0 aLaboratory colonisation of Aedes geniculatus c21/01/2016 a1-40 v343 aAedes geniculatus (Diptera: Culicidae) is a container-breeding mosquito species widespread throughout Europe and adjacent regions. Similar to other container-breeding mosquitoes, this primarily mammophilic species could be a vector for several pathogens of medical or veterinary importance. We here report for the first time a laboratory colonisation of Ae. geniculatus. Eggs and larvae were collected from forest edges in the area around Zürich, Switzerland. The colony could be established by maintaining adults at room temperature in a large cage where mating occurred. Females took blood meals through a pig intestine membrane in a restricted space. After embryo development for 8 – 14 weeks at 10 °C, ‘hatching in installments’ was induced. Our established colony of Ae. geniculatus, now in its sixth generation, facilitates future studies on basic ecology and physiology of this species and its vector capacity traits. Journal of the European Mosquito Control Association 34: 1-4, 2016 10aAedes geniculatus10ablood-feeding10acolony10acontainer-breeder10apig intestine membrane1 aWagner, Stefanie1 aMathis, Alexander uhttps://e-m-b.myspecies.info/node/114702003nas a2200241 4500008004100000245013000041210006900171260001500240300001000255490000700265520128200272653001901554653001801573653001401591653001501605653001501620653001001635653002201645100001601667700001301683700002201696856004301718 2015 eng d00aBlood-feeding Diptera (Culicidae and Ceratopogonidae) in an urban park of the city of Vitoria-Gasteiz (Basque Country, Spain)0 aBloodfeeding Diptera Culicidae and Ceratopogonidae in an urban p c06/07/2015 a10-140 v333 aHaematophagous and synanthropic Diptera are responsible for the transmission of several pathogenic agents to humans, domestic and wild animals, causing diverse vector-borne diseases of paramount importance. The presence of these blood-feeding insects is well reported in many areas of the world; however, the distribution in anthropogenic zones has not been studied so far across Spain. In order to gather information on this entomofauna in urban environments, adult stages were collected using an automatic entomological aspirator during the summer season in street lamps of a public park located in the city of Vitoria-Gasteiz (Basque Country, Spain). A total of 333 culicids from four species were collected in a two-year sampling period: Culex pipiens (91.3%), Culiseta longiareolata (8.1%), Culiseta litorea (0.3%) and Culex hortensis hortensis (0.3%). Moreover, 225 Culicoides specimens belonging to six different species were trapped: Culicoides punctatus (61.3 %), Culicoides obsoletus s.s. (16.8%), Culicoides duddingstoni (13.3%), Culicoides kibunensis (6.2%), Culicoides dewulfi (1.7%) and Culicoides clastrieri (0.4%). The first data of Culicoides species distribution in urban areas and the first record of C. litorea are recorded for the Basque Country region. 10aBasque Country10abiting midges10aCulicidae10aCulicoides10amosquitoes10aSpain10aurban environment1 aGonzalez, M1 aLopez, S1 aAlarcon-Elbal, PM uhttps://e-m-b.myspecies.info/node/114602131nas a2200229 4500008004100000245011000041210006900151260001300220300000800233490000700241520139800248653002401646653003901670653002101709653001801730653002301748653002801771653001301799100003001812700001601842856004301858 2015 eng d00aInfluence of five different larval control agents on oviposition of Culex pipiens L. (Diptera: Culicidae)0 aInfluence of five different larval control agents on oviposition c19/05/15 a5-90 v333 a
The effects of five different and widely used mosquito control agents (monomolecular film [MMF], diflubenzuron, Bacillus thuringiensis var. israelensis [Bti], Bacillus sphaericus [Bs] and temephos) on Culex pipiens L. oviposition were studied in both field and laboratory conditions. Half of the standard application dosages were used for all five control agents. Results were evaluated weekly for three weeks. All tested mosquito populations (Serik, Avariz, Slab and field) showed different degrees of oviposition preferences on experimental and control cups and pools. Culex pipiens L. tended to lay a lower number of eggs for tests groups than the control group throughout the whole test duration. Oviposition preferences of all Cx. pipiens decreased in the second week (except temephos both Avariz and field test, MMF both control and field test, Bs both Serik and control) and increased again in the third week. The repellent effects of MMF and temephos on oviposition were found to be higher than for the other larvicides. Mosquito species use different chemical cues for finding an ovipositional site. Therefore MMF and temephos may help in future control efforts, albeit less than the diflubenzuron, Bti and Bs on mosquitoes and mosquito-borne disease epidemics.
Aedes (Stegomya) albopictus is an invasive mosquito native to tropical areas in Southeast Asia. It is an important vector of several pathogens of public and veterinary health significance. In 2004 this mosquito was recorded for the first time in Spain (Catalonia) and detected in Majorca (Balearic Islands) in 2012. In this paper, we report for the first time the presence of Ae. albopictus on the island of Ibiza (‘Eivissa’, Balearic Islands, Spain). The high volume of goods arriving by maritime transport and the increase of commercial trade and visitors during recent years have been identified as the most probable entry routes for the species on the Island.
Mosquitoes found hibernating in underground shelters were studied in western Bohemia, Czech Republic. Species collected included Culiseta alaskaensis, Cs. annulata, Cs. glaphyroptera, Culex spp., and Anopheles maculipennis s.l.. Culex spp. were the most common, followed by Cs. glaphyroptera and Cs. annulata with few records of Cs. alaskaensis and An. maculipennis s.l.. Culex spp. were found in a variety of shelters, An. maculipennis s.l. preferred cellars, and Cs. alaskaensis where they were found, exhibited little preference. Culiseta annulata and Cs. glaphyroptera were found in similar numbers of cellars, caves, and bunkers, but Cs. glaphyroptera were found more commonly in mines than Cs. annulata.
During routine surveillance for invasive mosquitoes in southern Germany, using BG-Sentinel traps that were run continuously for one to two weeks, specimens were often damaged which prevented morphological species identification. We used DNA sequencing of the COI gene for molecular identification of damaged specimens, and observed two individuals from 2013 with aberrant COI-sequences. These two specimens were collected on separate occasions, and represent COI haplotypes that differ from Aedes (Finlaya) geniculatus by 2.0 - 2.4%, and from Aedes (Finlaya) echinus by 4.4 - 5.0%. Further analysis revealed a similarity to an Aedes (Finlaya) specimen from Macedonia, with these three specimens forming a genetic cluster. Our findings are discussed in the context of the three Aedes (Finlaya) species presently reported in Europe, and the potential introduction into Germany by road traffic.