TY - JOUR T1 - First finding of the West Nile virus vector Culex modestus Ficalbi 1889 (Diptera; Culicidae) in Sweden JF - Journal of the European Mosquito Control Association Y1 - 2018 A1 - Lindström, Anders A1 - Lilja, Tobias SP - 1 EP - 2 KW - Culex modestus KW - Mosquito KW - Sweden VL - 36 ER - TY - JOUR T1 - History of human-biting Culex pipiens in Sweden and Scandinavia JF - Journal of the European Mosquito Control Association Y1 - 2017 A1 - Lindström, Anders SP - 10 EP - 12 KW - Culex pipiens molestus KW - Culex pipiens s.l. KW - history KW - Scandinavia. KW - taxonomy AB - Journal of the European Mosquito Control Association 35: 10-12, 2017 VL - 35 ER - TY - JOUR T1 - Species identification of Swedish mosquitoes through DNA metabarcoding JF - Journal of the European Mosquito Control Association Y1 - 2017 A1 - Lilja, Tobias A1 - Nylander, Johan A.A A1 - Troell, Karin A1 - Lindström, Anders SP - 1 EP - 9 KW - COI KW - Culicidae KW - metabarcoding KW - next generation sequencing KW - surveillance KW - vectors AB - Abstract: DNA-barcoding utilises a fragment of the mitochondrial cytochrome oxidase subunit 1 (COI) gene to identify most animal species. Using next generation sequencing (NGS), this method can be further developed into metabarcoding processes that allow the simultaneous identification of several species from a mixed sample. We created a database of COI sequences of 27 mosquito species collected in Sweden, and combined our data with 27 additional sequences from GenBank to cover the taxa recently documented in Sweden and to include possible invasive taxa. Comparisons show that COI metabarcoding reliably identifies 41 of 54 species and the remainder to species group. Using three independent primer pairs along the COI gene, we further developed this barcoding approach to simultaneously identify Swedish mosquitoes in communities using NGS and quantify relative abundance of each mosquito species in the sample, using bioinformatics methods. We tested the accuracy of the metabarcoding method using communities assembled from morphologically identified mosquitoes, revealing 80% positive identification rate and the estimates of population structure which reflects the input sample. We conclude that metabarcoding is useful as a high throughput identification technique and for the quantification of species. Journal of the European Mosquito Control Association 35: 1-9, 2017 VL - 35 ER - TY - JOUR T1 - The geographic distribution of mosquito species in Sweden JF - Journal of the European Mosquito Control Association Y1 - 2013 A1 - Lundström, Jan O. A1 - Schäfer, Martina L. A1 - Hesson, Jenny C. A1 - Blomgren, Eric A1 - Lindström, Anders A1 - Wahlqvist, Pernilla A1 - Halling, Arne A1 - Hagelin, Anna A1 - Ahlm, Clas A1 - Evander, Magnus A1 - Broman, Tina A1 - Forsman, Mats A1 - Persson Vinnersten, Thomas Z. SP - 21 EP - 35 KW - CDC Light Trap KW - Counter-flow trap KW - Culicidae KW - mosquito distribution KW - mosquito surveillance KW - Sweden AB -

Surveillance of the actual distribution of mosquito species in Northern Europe is fundamental for evaluating risk for emerging pathogens, and for research on potential vectors. The Swedish mosquito fauna composition and geographic distribution, originally described by Professor Christine Dahl in the 1970´s, included 43 species. We have compiled the information published from 1978 to 2012, and our own surveillance data from 2001 to 2013, and compared this with the species list and geographic distribution provided in “Taxonomy and geographic distribution of Swedish Culicidae” by Dahl (1977). New species detected during these 36 years were Culiseta (Culicella) ochroptera (Peus, 1935) published 1984, Aedes (Aedes) rossicus Dolbeskin, Goritzkaja & Mitrofanova, 1930 published 1986, Anopheles (Anopheles) beklemishevi published 1986, Aedes (Ochlerotatus) euedes (Howard, Dyar & Knab, 1912) published 2001, Aedes (Ochlerotatus) nigrinus (Eckstein, 1918) first recorded in 2012, and Anopheles (Anopheles) algeriensis Theobald, 1903, first recorded in 2013. We provide maps with the distribution by province for each species, including historic information up until 1977, and new records from 1978 to 2013, showing the similarities and differences between the old and the new records. Important findings in recent years include the wide distribution of the Sindbis virus enzootic vector Culex (Culex) torrentium Martinii, 1925, and the more limited distribution of the potential West Nile virus vector Culex (Culex) pipiens Linnaeus, 1758. The updated list of mosquito species in Sweden now includes 49 species.

VL - 31 ER -