COI sequencing for invasive mosquito surveillance in Germany reveals genetically divergent specimens near Aedes geniculatus (Diptera: Culicidae)

Publication Type:Journal Article
Year of Publication:2014
Authors:Krüger, A, Obermayr, U, Czajka, C, Bueno-Marí, R, Jost, A, Rose, A
Journal:Journal of the European Mosquito Control Association
Volume:32
Pagination:22-26
Date Published:22/10/14
Keywords:Aedes (Finlaya) echinus, Aedes (Finlaya) geniculatus, Aedes (Finlaya) gilcolladoi, COI barcoding, Germany, invasive species
Abstract:

<p>During routine surveillance for invasive mosquitoes in southern Germany, using BG-Sentinel traps that were run continuously for one to two weeks, specimens were often damaged which prevented morphological species identification. We used DNA sequencing of the COI gene for molecular identification of damaged specimens, and observed two individuals from 2013 with aberrant COI-sequences. These two specimens were collected on separate occasions, and represent COI haplotypes that differ from Aedes (Finlaya) geniculatus by 2.0 - 2.4%, and from Aedes (Finlaya) echinus by 4.4 - 5.0%. Further analysis revealed a similarity to an Aedes (Finlaya) specimen from Macedonia, with these three specimens forming a genetic cluster. Our findings are discussed in the context of the three Aedes (Finlaya) species presently reported in Europe, and the potential introduction into Germany by road traffic.</p>
<p><br />
&nbsp;</p>

File attachments: 
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith